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pYTRW27K_7Ti1
(Plasmid #177294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177294 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTRW22K_7Ti1
  • Backbone size w/o insert (bp) 13522
  • Vector type
    Bacterial Expression, Synthetic Biology ; Yeast Expression, rrn genomic integration
  • Selectable markers
    URA3 ; Tetracycline

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PsacB-sacB
  • Alt name
    sucrose sensitivity module
  • Alt name
    Levansucrase
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    1884

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGATTGACGGCATTACG
  • 3′ sequencing primer GTCTGCGTAGAATCCTC, TTAAAGACGCGGCCAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Schaefer et al (pK19-mobSacB in PMID: 8045426)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Part of the pYT vector series which facilitates chromosomal interposon integration in rrn "landing pads" with selection on tetracycline and transcriptional reporter LacZ (https://www.uniprot.org/uniprot/P00722; methods: blue/ white selection, ONPG-assay). Target genes can be inserted at the I-SceI site. The vector is equipped with SacB for counter selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYTRW27K_7Ti1 was a gift from Karl-Erich Jaeger (Addgene plasmid # 177294 ; http://n2t.net/addgene:177294 ; RRID:Addgene_177294)

  • For your References section:

    The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies S, Loeschcke A. FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. eCollection 2023. 10.1093/femsmc/xtac030 PubMed 37333445
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