pEcMetRSH6
(Plasmid
#177593)
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PurposeExpresses His tagged MetRS in Escherichia coli cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJL-H6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMethionine tRNA Synthetase
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SpeciesEscherichia coli
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Entrez GenemetG (a.k.a. b2114, ECK2107)
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Tag
/ Fusion Protein
- Hexahistidine (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEcMetRSH6 was a gift from Kambiz Hamadani (Addgene plasmid # 177593 ; http://n2t.net/addgene:177593 ; RRID:Addgene_177593)