PurposeMammalian expression of green fluorescent potassium indicator KRaION1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||177801||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4005
- Total vector size (bp) 5178
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)1173
- Promoter CMV
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-Fw (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://doi.org/10.1101/2021.10.07.463355 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKRaION1-N1 was a gift from Edward Boyden (Addgene plasmid # 177801 ; http://n2t.net/addgene:177801 ; RRID:Addgene_177801)
For your References section:Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium Indicators through Structure-Guided and Genome Mining Strategies. Torres Cabán CC, Yang M, Lai C, Yang L, Subach FV, Smith BO, Piatkevich KD, Boyden ES. ACS Sens. 2022 May 27;7(5):1336-1346. doi: 10.1021/acssensors.1c02201. Epub 2022 Apr 15. 10.1021/acssensors.1c02201 PubMed 35427452