pCDH-SFFV-GLuc-CreERT2-mCherry
(Plasmid
#178185)
-
Purposethe plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCDH
-
Backbone manufacturerSystem Biosciences
- Total vector size (bp) 9792
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameGLuc
-
Insert Size (bp)797
Gene/Insert 2
-
Gene/Insert nameCreERT2
-
Insert Size (bp)1970
Gene/Insert 3
-
Gene/Insert namemCherry
-
Insert Size (bp)681
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCDH-SFFV-GLuc-CreERT2-mCherry was a gift from Irmela Jeremias (Addgene plasmid # 178185 ; http://n2t.net/addgene:178185 ; RRID:Addgene_178185) -
For your References section:
In vivo inducible reverse genetics in patients' tumors to identify individual therapeutic targets. Carlet M, Volse K, Vergalli J, Becker M, Herold T, Arner A, Senft D, Jurinovic V, Liu WH, Gao Y, Dill V, Fehse B, Baldus CD, Bastian L, Lenk L, Schewe DM, Bagnoli JW, Vick B, Schmid JP, Wilhelm A, Marschalek R, Jost PJ, Miething C, Riecken K, Schmidt-Supprian M, Binder V, Jeremias I. Nat Commun. 2021 Sep 27;12(1):5655. doi: 10.1038/s41467-021-25963-z. 10.1038/s41467-021-25963-z PubMed 34580292
Map uploaded by the depositor.
