pBTK800
(Plasmid
#179209)
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PurposeGFP dropout for assembling a double-stranded RNA expression plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneRSF1010
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP dropout
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SpeciesSynthetic
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Insert Size (bp)1012
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK800 was a gift from Jeffrey Barrick (Addgene plasmid # 179209 ; http://n2t.net/addgene:179209 ; RRID:Addgene_179209) -
For your References section:
Honey bee functional genomics using symbiont-mediated RNAi. Lariviere PJ, Leonard SP, Horak RD, Powell JE, Barrick JE. Nat Protoc. 2023 Mar;18(3):902-928. doi: 10.1038/s41596-022-00778-4. Epub 2022 Dec 2. 10.1038/s41596-022-00778-4 PubMed 36460809
