pFastBac1-Nucleocapsid
(Plasmid #179696)

• Purpose: Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
• Depositing Lab: Mark Arbing
• Publication: Expression of His6 tagged SARS CoV-2 nucleocapsid in Sf9 cells (unpublished)

Backbone

• Vector backbone: pFastBac1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4760
• Total vector size (bp): 6067
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 Nucleocapsid (N protein)
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 1200
• GenBank ID: NC_045512.2
• Entrez Gene: N (a.k.a. GU280_gp10)
• Promoter: polyhedrin
• Tag / Fusion Protein:
  • His6-TEV protease site (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: polyhedrin forward
• 3′ sequencing primer: polyhedrin reverse

Resource Information

• Supplemental Documents:
  • pfastbac1-nucleocapsid.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expresses SARS CoV-2 Nucleoprotein (N gene; nucleocapsid) in insect cells with TEV-cleavable N-terminal His6 tag. Express 4-5 days at 27C. Yield from NiNTA column is approximately 100 mg/L of culture using ExpiSF expression system.