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TtEnd_H280A_pEHISTEV
(Plasmid #179735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179735 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEHISTEV vector
  • Backbone manufacturer
    BSRC, St Andrews
  • Backbone size w/o insert (bp) 5363
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TtEnd5A
  • Alt name
    TtCel5A
  • Species
    Thermogutta terrifontis R1
  • Insert Size (bp)
    1539
  • Mutation
    His280 has been mutated to Ala280.
  • GenBank ID
    CP018477.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TtEnd_H280A_pEHISTEV was a gift from James Naismith (Addgene plasmid # 179735 ; http://n2t.net/addgene:179735 ; RRID:Addgene_179735)

  • For your References section:

    Structural and functional snapshots of a broad-specificity endoglucanase from Thermogutta terrifontis for biomass saccharification. Hussain N, Mikolajek H, Harrison PJ, Paterson N, Akhtar MW, Sadaf S, Naismith JH. Arch Biochem Biophys. 2025 Feb;764:110274. doi: 10.1016/j.abb.2024.110274. Epub 2024 Dec 17. 10.1016/j.abb.2024.110274 PubMed 39701201
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