PurposeDonor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||179892||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5098
Vector typeMammalian Expression, Bacterial Expression, CRISPR ; Donor for homologous based knock in
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert nameRAB11A, member RAS oncogene family
SpeciesH. sapiens (human)
Insert Size (bp)1898
Entrez GeneRAB11A (a.k.a. YL8)
- Promoter SFFV
- Cloning method Gibson Cloning
- 5′ sequencing primer CGACTCACTATAGGGAGAGCG
- 3′ sequencing primer AAGAACATCGATTTTCCATGGCAG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Here donor for integration of SFFV-T2A-Puromycine resistance-PolyA cassette (for knock out) to Rab11a locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11a expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJET-61-SFFV-T2A-PuroR-polyA-R11a was a gift from Patrick Caswell (Addgene plasmid # 179892 ; http://n2t.net/addgene:179892 ; RRID:Addgene_179892)
For your References section:On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members. Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT. Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651. 10.7554/eLife.76651 PubMed 35708998