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On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members.
Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
179887pCDH-EF1a-HygroR-T2A-TetOn3GExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)
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179888pCDH-EF1a-mTagBFP-T2A-TetOn3GExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)
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179889pCDH-AtAFB2-mTagBFP2Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)
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179890pJET-07-mNeonGreen-R11aDonor with homologous arms for Rab11a to knock in mNeonGreen
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179891pJET-10-mCherry-R11bDonor with homologous arms for Rab11b to knock in mCherry
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179892pJET-61-SFFV-T2A-PuroR-polyA-R11aDonor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out
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179893pJET-62-SFFV-T2A-PuroR-polyA-R11bDonor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)
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179894pJET-63-SFFV-T2A-BlaR-polyA-R11aDonor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)
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179895pJET-64-SFFV-T2A-BlaR-polyA-R11bDonor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)
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179896pMKRQ_11_DExCon-mNeonGreen-R25Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module
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179897pJET-22-DExCon-mNeonGreen-R11aDonor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module
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179898pJET-23-DExCon-mCherry-R11bDonor with homologous arms for Rab11b to knock in DExCon-mCherry module
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179899pJET-67-DExCon-mNeonGreen-R11bDonor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module
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179900pJET-59-DExCon-mTagBFP2-R25Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module
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179901pJET-41-DExCon-antiGFPnanobody-mCherry-R11bDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module
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179902pJET-42-DExCon-antiGFPnanobody-mCherry-R11aDonor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module
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179903pJET-49-DExogron-mNeonGreen-R11aDonor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen module
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179904pJET-50-DExogron-mCherry-R11bDonor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module
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Antibodies from Article