Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17991||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6620
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsDH5a, HB101
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1300
Entrez GeneWnt1 (a.k.a. Int-1, Wnt-1, sw, swaying)
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATCG
- 3′ sequencing primer ACCTACAGGTGGGGTCTTTCATTCCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Fung, Y.K., Shackleford, G. M., Brown, A. M., Sanders, G.S., and Varmus, H. E. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol., 5:3337-3344, 1985.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLNC Wnt-1 was a gift from Jan Kitajewski (Addgene plasmid # 17991 ; http://n2t.net/addgene:17991 ; RRID:Addgene_17991)
For your References section:Transformation by Wnt family proteins correlates with regulation of beta-catenin. Shimizu H, Julius MA, Giarre M, Zheng Z, Brown AM, Kitajewski J. Cell Growth Differ. 1997 Dec . 8(12):1349-58. PubMed 9419423