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pCAG-HP1γ-I165E-EGFP
(Plasmid #179929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179929 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG-eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cbx 3
  • Alt name
    HP1γ
  • Alt name
    HP1g
  • Species
    M. musculus (mouse)
  • Mutation
    I165E
  • GenBank ID
    NM_016587
  • Entrez Gene
    Cbx3 (a.k.a. HP1g, M32)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CAG-FW
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HP1γ-I165E-EGFP was a gift from Andrew Newman (Addgene plasmid # 179929 ; http://n2t.net/addgene:179929 ; RRID:Addgene_179929)

  • For your References section:

    Glial reactivity and cognitive decline follow chronic heterochromatin loss in neurons. Newman AG, Sharif J, Bessa P, Zaqout S, Brown JP, Richter D, Dannenberg R, Nakayama M, Mueller S, Schaub T, Manickaraj S, Boehm-Sturm P, Ohara O, Koseki H, Singh PB, Tarabykin V. Nat Commun. 2025 Aug 8;16(1):7325. doi: 10.1038/s41467-025-61319-7. 10.1038/s41467-025-61319-7 PubMed 40781223
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