Px330-EGFP-LRRK2-CRISPR/Cas9
(Plasmid #180437)

• Purpose: Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells
• Depositing Lab: Frank Soldner
• Publication: Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208.

Backbone

• Vector backbone: pX330-EGFP
• Backbone manufacturer: Jaenisch Laboratory, Soldner et al. Nature (2016) vol 533, pg 95-99
• Backbone size w/o insert (bp): 10200
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA targeting LRRK2-G2019S
• Alt name: PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• gRNA/shRNA sequence: GATTGCAAAGATTGCTGACTA
• Species: H. sapiens (human)
• Insert Size (bp): 21
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: ACTATCATATGCTTACCGTAAC

Resource Information

• Supplemental Documents:
  • Px330-EGFP-LRRK2-CRISPR_Cas9.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry