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Highly efficient generation of isogenic pluripotent stem cell models using prime editing.
Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F
Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
180014AAVS1-SA-neo-CAGGS-PE2-2A-GFPTargeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus
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180015pET30a(+)-PE2Expressing PE2 protein in E.coli for protein purification
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180016pU6-pegRNA-SNCA-A30PTransiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells
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180017pU6-pegRNA-HEK3-CTTins-px330-scaffoldTransiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.
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180018pBPK1520-SNCA-A30P-ngTransiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach
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180019pBPK1520-HEK3-CTTins-ngTransiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach
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180432pU6-pegRNA-LRRK2-G2019S-3aTransiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells
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180433pU6-pegRNA-LRRK2-G2019S-3bTransiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells
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180434pU6-pegRNA-LRRK2-G2019S-3cTransiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells
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180435pU6-pegRNA-LRRK2-G2019S-3dTransiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells
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180436BPK1520-LRRK2-G2019S-ngTransiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach
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180437Px330-EGFP-LRRK2-CRISPR/Cas9Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells
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181738pU6-pegRNA-SNCA-A53TTransiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells
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181739pBPK1520-SNCA-A53T-ngTransiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach
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Recombinant Antibodies from Article