pCMV-Armc12/FLAG/BioID2/HA
(Plasmid #180498)

• Purpose: Expression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
• Depositing Lab: Masahito Ikawa
• Publication: Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 10.1073/pnas.2018355118.

Backbone

• Vector backbone: MCS-BioID2-HA
• Backbone size w/o insert (bp): 6112
• Total vector size (bp): 6112
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: armadillo repeat containing 12
• Alt name: Armc12
• Species: M. musculus (mouse)
• Insert Size (bp): 1020
• GenBank ID: NM_026290.3
• Entrez Gene: Armc12 (a.k.a. 4930511I11Rik, AV041658)
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • FLAG tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTAATACGACTCACTATAGG
• 3′ sequencing primer: AGCTCACGTTCCACTCCTTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry