pαH-S-GSAS-2P-OMICRON
(Plasmid #180593)

• Purpose: Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
• Depositing Lab: Priyamvada Acharya
• Publication: Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6. doi: 10.1016/j.molcel.2022.03.028. Epub 2022 Mar 25.

Backbone

• Vector backbone: pαH
• Backbone size w/o insert (bp): 4746
• Total vector size (bp): 8361
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Spike (S-GSAS-2P-OMICRON)
• Alt name: pαH-S-GSAS-2P-OMICRON
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 3615
• Mutation: Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; A67V-∆H69/V70-T95I, G142D, V143-, Y144-, Y145-, N211-, L212I, +214EPE-G339D-S371L-S373P-S375F-K417N-N440K-G446S-S477N-T478K-E484A-Q493R-G496S-Q498R-N501Y-Y505H-T547K-D614G-H655Y-N679K-P681H-N764K -D796Y-N856K-Q954H-N969K-K986P-V987P-L981F
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Tag / Fusion Protein:
  • HRV3C cleavage site, 8X His tag, 2X Strep-Tag II (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pαH-S-GSAS-2P-OMICRON.gbk
• A portion of this plasmid was derived from a plasmid made by: Jason McLellan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See this as a reference: https://www.addgene.org/164566/.

Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.