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PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
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Depositing Lab
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Depositing OrganizationUniversity of Texas at Austin
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 154754-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 154754-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepαH
- Backbone size w/o insert (bp) 4746
- Total vector size (bp) 8370
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpike (HexaPro variant)
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Insert Size (bp)3624
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MutationEctodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P
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GenBank IDMN908947
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- HRV 3C cleavage site (before tags) (C terminal on backbone)
- 8X His tag (C terminal on backbone)
- 2X Strep-Tag II (C terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 154754-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 154754-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SARS-CoV-2 S HexaPro was a gift from Jason McLellan (Addgene plasmid # 154754 ; http://n2t.net/addgene:154754 ; RRID:Addgene_154754) -
For your References section:
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Hsieh CL, Goldsmith JA, Schaub JM, DiVenere AM, Kuo HC, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou CW, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS. Science. 2020 Sep 18;369(6510):1501-1505. doi: 10.1126/science.abd0826. Epub 2020 Jul 23. 10.1126/science.abd0826 PubMed 32703906
Map uploaded by the depositor.
