pEUK037
(Plasmid
#180829)
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PurposeT7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 6684
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCnGdmB
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Alt nameCNE_RS35790
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SpeciesCupriavidus necator N-1
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Insert Size (bp)981
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Entrez GeneCNE_RS35790 (a.k.a. CNE_RS35790, CNE_BB1p12290)
- Promoter T7
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Tag
/ Fusion Protein
- 6X His (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEUK037 was a gift from Gregg Beckham (Addgene plasmid # 180829 ; http://n2t.net/addgene:180829 ; RRID:Addgene_180829) -
For your References section:
Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation. Bleem A, Kuatsjah E, Presley GN, Hinchen DJ, Zahn M, Garcia DC, Michener WE, König G, Tornesakis K, Allemann MN, Giannone RJ, McGeehan JE, Beckham GT, Michener JK. Chem Catalysis, 2022 10.1016/j.checat.2022.04.019