pRQ-Id1HA
(Plasmid #18112)

• Depositing Lab: Alex Swarbrick
• Publication: Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Generated from multiple sources
• Backbone size w/o insert (bp): 6633
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: STBL2 @ 30'C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Inhibitor of differentiation 1
• Alt name: id1
• Alt name: idb1
• Species: M. musculus (mouse)
• Insert Size (bp): 443
• GenBank ID: NM_010495
• Entrez Gene: Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: gateway (unknown if destroyed)
• 3′ cloning site: Gateway (unknown if destroyed)
• 5′ sequencing primer: GCT CGT TTA GTG AAC CGT CA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry