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pcDNA3 mId1
(Plasmid #16060)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16060 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Id1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140, D2Wsu140e, Idb, Idb1, bHLHb2, bHLHb24)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

mId1 protein coding sequence was amplified from a lab stock plasmid by high fidelity PCR. EcoRI and BamHI restriction sites were added to the 5' and 3' ends, respectively. This fragment was first cloned into EcoRI and BamHI sites of pBluescript II KS (-), then the HindIII-NotI fragment from that plasmid was subcloned into HindIII and NotI sites of pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 mId1 was a gift from Robert Benezra (Addgene plasmid # 16060 ; http://n2t.net/addgene:16060 ; RRID:Addgene_16060)