|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16060||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)500
Entrez GeneId1 (a.k.a. AI323524, D2Wsu140, D2Wsu140e, Idb, Idb1, bHLHb2, bHLHb24)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
mId1 protein coding sequence was amplified from a lab stock plasmid by high fidelity PCR. EcoRI and BamHI restriction sites were added to the 5' and 3' ends, respectively. This fragment was first cloned into EcoRI and BamHI sites of pBluescript II KS (-), then the HindIII-NotI fragment from that plasmid was subcloned into HindIII and NotI sites of pcDNA3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 mId1 was a gift from Robert Benezra (Addgene plasmid # 16060 ; http://n2t.net/addgene:16060 ; RRID:Addgene_16060)