pKOR1-mcs
(Plasmid
#181755)
-
Purpose(Empty Backbone) Allele replacement for Staphylococcus aureus
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepKOR1
-
Backbone manufacturerTaeok Bae
- Backbone size (bp) 10030
-
Modifications to backboneReplacing the lambda recombination cassette and the cat (-) and ccdB genes of pKOR1 with a conventional multiple cloning site (cloned in ApaI/KpnI).
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Dr. Suzan Rooijakkers at the University Medical Center Utrecht, the Netherlands modified pKOR1 to generate pKOR1-mcs.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKOR1-mcs was a gift from Suzan Rooijakkers (Addgene plasmid # 181755 ; http://n2t.net/addgene:181755 ; RRID:Addgene_181755) -
For your References section:
Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors. Stapels DA, Ramyar KX, Bischoff M, von Kockritz-Blickwede M, Milder FJ, Ruyken M, Eisenbeis J, McWhorter WJ, Herrmann M, van Kessel KP, Geisbrecht BV, Rooijakkers SH. Proc Natl Acad Sci U S A. 2014 Sep 9;111(36):13187-92. doi: 10.1073/pnas.1407616111. Epub 2014 Aug 26. 10.1073/pnas.1407616111 PubMed 25161283
