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Addgene

Fc-E3
(Plasmid #181876)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181876 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSEC-Tag
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fc (murine) fused to NOTCH3 (human EGF-like domains 1-3)
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    NOTCH3 (a.k.a. CADASIL, CADASIL1, CASIL, IMF2, LMNS)
  • Promoter CMV
  • Tag / Fusion Protein
    • Fc murine IgG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer custom
  • 3′ sequencing primer custom
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fc-E3 was a gift from Michael Wang (Addgene plasmid # 181876 ; http://n2t.net/addgene:181876 ; RRID:Addgene_181876)

  • For your References section:

    Hydrolysis of a second Asp-Pro site at the N-terminus of NOTCH3 in inherited vascular dementia. Zhang X, Lee SJ, Wang MM. Sci Rep. 2021 Aug 26;11(1):17246. doi: 10.1038/s41598-021-96679-9. 10.1038/s41598-021-96679-9 PubMed 34446744
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