Linked mRuby3 VLP
(Plasmid #182430)

• Purpose: Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
• Depositing Lab: Claudia Vickers
• Publication: Li Chen Cheah et al BioRxiv, 2022

Backbone

• Vector backbone: pILGFPB5A
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VP2C-GFP-mRuby3
• Alt name: VP2C-yEGFP-mRuby3, VP2C-GFP-RFP
• Species: Synthetic; Murine polyomavirus
• Insert Size (bp): 1665
• Promoter: GAL10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGTAATGCCATGTAATATGATTATTAAAC
• 3′ sequencing primer: GCATTGGCACGGTGCAACAC

Gene/Insert 2

• Gene/Insert name: deltaVP1
• Alt name: VP1, deltaNLS VP1
• Species: Synthetic; Murine polyomavirus
• Insert Size (bp): 1143
• Promoter: GAL1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACTTCTTATTCAAATGTCATAAAAGTATCAAC
• 3′ sequencing primer: CCAGCCTGCTTTTCTGTAAC

Resource Information

• Supplemental Documents:
  • Linked VLP mRuby3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mRuby3 is slow to mature at 30 degC. Further incubation at 37 degC required after protein purification for complete fluorophore maturation.

Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.