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Addgene

pLenti GW V5 Eco/Dam hum LaminB1
(Plasmid #182671)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 182671 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti Gateway
  • Backbone manufacturer
    10364
  • Backbone size w/o insert (bp) 8591
  • Modifications to backbone
    V5 - Dam
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lamin B1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1761
  • Entrez Gene
    LMNB1 (a.k.a. ADLD, LMN, LMN2, LMNB, MCPH26)
  • Promoter HSP
  • Tags / Fusion Proteins
    • E. Coli Dam (N terminal on insert)
    • V5 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti GW V5 Eco/Dam hum LaminB1 was a gift from Bas van Steensel (Addgene plasmid # 182671 ; http://n2t.net/addgene:182671 ; RRID:Addgene_182671)
  • For your References section:

    Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Guelen L, Pagie L, Brasset E, Meuleman W, Faza MB, Talhout W, Eussen BH, de Klein A, Wessels L, de Laat W, van Steensel B. Nature. 2008 Jun 12;453(7197):948-51. doi: 10.1038/nature06947. Epub 2008 May 7. 10.1038/nature06947 PubMed 18463634