Skip to main content
Addgene
Open Menu

pNL3.1-Erg
(Plasmid #182706)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182706 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 182706-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 182706-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pNL3.1[Nluc/minP]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3151
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhancer of Erythroblast transformation-specific-Related Gene
  • Alt name
    ERG enhancer
  • Alt name
    chr16:95509125-95510537
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    162
  • GenBank ID
    NM_001302152.1
  • Entrez Gene
    Erg (a.k.a. D030036I24Rik)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNL3.1-Erg was a gift from Robert Rowe (Addgene plasmid # 182706 ; http://n2t.net/addgene:182706 ; RRID:Addgene_182706)

  • For your References section:

    Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Wang D, Tanaka-Yano M, Meader E, Kinney MA, Morris V, Lummertz da Rocha E, Liu N, Liu T, Zhu Q, Orkin SH, North TE, Daley GQ, Rowe RG. Cell Rep. 2022 Apr 5;39(1):110587. doi: 10.1016/j.celrep.2022.110587. 10.1016/j.celrep.2022.110587 PubMed 35385744
Related items: