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alpha7nACHR_Nacho_mCherry
(Plasmid #182939)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 182939 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    synthesized
  • Vector type
    Mammalian Expression ; Synthesized

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NACH7
  • Alt name
    alpha7 nicotinic acetylcholine receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1508
  • GenBank ID
    U40583.2
  • Entrez Gene
    CHRNA7 (a.k.a. CHRNA7-2, NACHRA7)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer CCTCACATTGCCAAAAGACG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized based on Genbank sequence
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

alpha7 nicotinic acetylcholine receptor cloned upstream of IRES-Nacho sequence to allow alpha7 expression in mammalian cells with chaperone protein NACHO (TMEM35a). The plasmid contains CMV-mCherry to report transfected cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha7nACHR_Nacho_mCherry was a gift from Robert Brenner (Addgene plasmid # 182939 ; http://n2t.net/addgene:182939 ; RRID:Addgene_182939)
  • For your References section:

    Dequalinium chloride is an antagonists of alpha7 nicotinic acetylcholine receptors. Belanger-Coast MG, Zhang M, Bugay V, Gutierrez RA, Gregory SR, Yu W, Brenner R. Eur J Pharmacol. 2022 Jun 15;925:175000. doi: 10.1016/j.ejphar.2022.175000. Epub 2022 May 4. 10.1016/j.ejphar.2022.175000 PubMed 35525312