|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1831||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3200
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namewingless related MMTV integration site 10b
Alt namebeta-actin promoter
SpeciesM. musculus (mouse)
Insert Size (bp)3400
Entrez GeneWnt10b (a.k.a. Wnt1, Wnt12)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
See scanned map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BA-Wnt10b was a gift from Philip Leder (Addgene plasmid # 1831 ; http://n2t.net/addgene:1831 ; RRID:Addgene_1831)
For your References section:Wnt-10b directs hypermorphic development and transformation in mammary glands of male and female mice. Lane TF, Leder P. Oncogene 1997 Oct;15(18):2133-44. 10.1038/sj.onc.1201593 PubMed 9393971
Map uploaded by the depositor.