pACT-Lex
(Plasmid
#183149)
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PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACT2
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Backbone manufacturerClontech
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Vector typeYeast Expression ; Yeat two-hybrid
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGAL4 AD
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- HA tag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACT-Lex was a gift from Assaf Mosquna (Addgene plasmid # 183149 ; http://n2t.net/addgene:183149 ; RRID:Addgene_183149) -
For your References section:
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions. Feuer E, Zimran G, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. 10.1021/acssynbio.2c00192 PubMed 35895499
Map uploaded by the depositor.
