A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions.
Feuer E, Zimran G, Shpilman M, Mosquna A
ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. PubMed Article
Feuer E, Zimran G, Shpilman M, Mosquna A
ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183147 | pHO-UAS-mScarlet-I | Yeat two-hybrid fluorecent reporter for genomic integration |
| 183148 | pBD-pZ-FLAG | Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM) |
| 183149 | pACT-Lex | Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112) |
| 183150 | p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM | Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM) |