p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
(Plasmid
#183150)
-
PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneHO-Poly-KanMX4-HO
-
Backbone manufacturerVoth et al., 2001
-
Vector typeFor yeast genomic integration
-
Selectable markersNourseothricin (cloNAT)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert 1
-
Gene/Insert nameLex-ER-B112
-
SpeciesSynthetic
Gene/Insert 2
-
Gene/Insert nameZif268 DBD - hPRLBD - MSN2AD
-
Alt nameZPM
-
SpeciesSynthetic
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Digest with PvuII for obtaining linear fragment for genomic integration to yeast into site 20 (Flagfeldt et al., 2009) via homologous recombination.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM was a gift from Assaf Mosquna (Addgene plasmid # 183150 ; http://n2t.net/addgene:183150 ; RRID:Addgene_183150) -
For your References section:
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein-Protein Interactions. Feuer E, Zimran G, Shpilman M, Mosquna A. ACS Synth Biol. 2022 Jul 27. doi: 10.1021/acssynbio.2c00192. 10.1021/acssynbio.2c00192 PubMed 35895499
Map uploaded by the depositor.
