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Addgene

JBL6420
(Plasmid #183857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183857 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli thiB TPP Riboswitch Rescue Invader A regulated sfGFP
  • Species
    E. coli
  • Promoter J23119 - Anderson Promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Spe1/J23119 Promoter + TPP thiB E. coli Riboswitch + INV RES 5 + sfGFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JBL6420 was a gift from Julius Lucks (Addgene plasmid # 183857 ; http://n2t.net/addgene:183857 ; RRID:Addgene_183857)

  • For your References section:

    A transient intermediate RNA structure underlies the regulatory function of the E. coli thiB TPP translational riboswitch. Berman KE, Steans R, Hertz LM, Lucks JB. RNA. 2023 Nov;29(11):1658-1672. doi: 10.1261/rna.079427.122. Epub 2023 Jul 7. 10.1261/rna.079427.122 PubMed 37419663
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