pSLQ10873
(Plasmid #183961)

• Purpose: U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
• Depositing Lab: Stanley Qi
• Publication: Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12.

Backbone

• Vector backbone: pUC
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: HyperdCas12a
• Alt name: LbdCas12a(D156R, E292R, D235R, D350R)
• Alt name: LbdCfp1(D156R, E292R, D235R, D350R)
• Species: Synthetic
• Mutation: D832A, D156R, E292R, D235R, D350R
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGCAAAGAATTCTGCAGTCGACGGTACC
• 3′ sequencing primer: TTTTGGCAGAGGGAAAAAGA

Gene/Insert 2

• Gene/Insert name: poly-crRNA array targeting Sox2-Klf4-Oct4
• Species: Synthetic
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGATACAAGGCTGTTAGAGAG
• 3′ sequencing primer: gcggccgcctaatggatccctcgag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry