Multiplexed genome regulation in vivo with hyper-efficient Cas12a.
Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A, Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. PubMed Article
Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A, Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183955 | pSLQ10333 | CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR |
| 183956 | pSLQ10844 | CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR |
| 183957 | pSLQ10704 | U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP |
| 183958 | pSLQ10713 | U6-crSox2-CAG-hyperdCas12a-miniVPR |
| 183959 | pSLQ10714 | U6-crKlf4-CAG-hyperdCas12a-miniVPR |
| 183960 | pSLQ10715 | U6-crOct4-CAG-hyperdCas12a-miniVPR |
| 183961 | pSLQ10873 | U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR |
| 183962 | pSLQ10871 | U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR |
| 183963 | pSLQ10875 | CBA-hyperdCas12a-HA-KRAB-mCherry |
| 183964 | pSLQ10878 | CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR |
| 183965 | crTet in pSLQ8453 | U6-crTet-EF1a-Puro-T2A-BFP-WPRE |