pT2EFCRET(#324)
(Plasmid #184059)

• Purpose: cyclofen-inducible CRE activation in zebrafish transient transgenic
• Depositing Lab: Sophie Vriz
• Publication: Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 10.1089/zeb.2009.0632.

Backbone

• Vector backbone: pT2KXIGdin
• Backbone manufacturer: K Kawakami (Kawakami et al, 2004, Dev Cell 7:133)
• Backbone size w/o insert (bp): 7263
• Vector type: Zebrafish transient transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRE-ERT2
• Species: Phage P1 (CRE), H. sapiens (ERT2)
• Insert Size (bp): 1998
• Mutation: ERT2: G400V, M543A, L544A, deleted 1-281;
• Promoter: Xl-EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PpuMI (not destroyed)
• 3′ cloning site: ClaI (destroyed during cloning)
• 5′ sequencing primer: xefprom
• 3′ sequencing primer: SVLrev34

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CRE: YP_006472; ERT2: NP_000116;