pCSTG4FER3(#190)
(Plasmid #184069)

• Purpose: cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis
• Depositing Lab: Sophie Vriz
• Publication: Vriz lab cyclofen-inducible ERT2 plasmids (unpublished)

Backbone

• Vector backbone: pCST (pCS2 derivative)
• Backbone manufacturer: M Volovitch
• Backbone size w/o insert (bp): 4092
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal4bdFF-ERT2
• Species: S. cerevisiae (Gal4bd), Herpes Simplex Virus (F), H. sapiens (ERT2)
• Insert Size (bp): 1506
• Mutation: ERT2: G400V, M543A, L544A, deleted 1-281;
• Promoter: sCMV+SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BsaBI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SVLrev34

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synthetic ERT2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gal4FF: AJD87368; ERT2: NP_000116;