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Addgene

pcDNA FZD4_5CRDlinker
(Plasmid #184260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184260 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1 mychis
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FZD4_5CRDlinker
  • Species
    M. musculus (mouse)
  • Mutation
    Replaced FZD4 aa41-203 with FZD5 aa27-221
  • Entrez Gene
    Fzd4 (a.k.a. Fz4)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA FZD4_5CRDlinker was a gift from Hee-Jung Choi (Addgene plasmid # 184260 ; http://n2t.net/addgene:184260 ; RRID:Addgene_184260)

  • For your References section:

    Functional role of the Frizzled linker domain in the Wnt signaling pathway. Ko SB, Mihara E, Park Y, Roh K, Kang C, Takagi J, Bang I, Choi HJ. Commun Biol. 2022 May 5;5(1):421. doi: 10.1038/s42003-022-03370-4. 10.1038/s42003-022-03370-4 PubMed 35513706
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