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Addgene

pcDNA FZD6_4CRDlinker
(Plasmid #184266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184266 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1 mychis
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FZD6_4CRDlinker
  • Species
    H. sapiens (human)
  • Mutation
    Replaced FZD6 aa19-184 with FZD4 aa41-203
  • Entrez Gene
    FZD6 (a.k.a. FZ-6, FZ6, HFZ6, NDNC1, NDNC10)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA FZD6_4CRDlinker was a gift from Hee-Jung Choi (Addgene plasmid # 184266 ; http://n2t.net/addgene:184266 ; RRID:Addgene_184266)

  • For your References section:

    Functional role of the Frizzled linker domain in the Wnt signaling pathway. Ko SB, Mihara E, Park Y, Roh K, Kang C, Takagi J, Bang I, Choi HJ. Commun Biol. 2022 May 5;5(1):421. doi: 10.1038/s42003-022-03370-4. 10.1038/s42003-022-03370-4 PubMed 35513706
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