3322_pETcon_SARS2_Omicron-BA2
(Plasmid
#184409)
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Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepETcon
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Vector typeYeast Expression
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Selectable markersTRP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD)
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SpeciesSARS-CoV-2 virus
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MutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N440K+S477N+T478K+E484A+Q493R+Q498R+N501Y+Y505H
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- c-Myc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3322_pETcon_SARS2_Omicron-BA2 was a gift from Jesse Bloom (Addgene plasmid # 184409 ; http://n2t.net/addgene:184409 ; RRID:Addgene_184409) -
For your References section:
Shifting mutational constraints in the SARS-CoV-2 receptor-binding domain during viral evolution. Starr TN, Greaney AJ, Hannon WW, Loes AN, Hauser K, Dillen JR, Ferri E, Farrell AG, Dadonaite B, McCallum M, Matreyek KA, Corti D, Veesler D, Snell G, Bloom JD. Science. 2022 Jun 28:eabo7896. doi: 10.1126/science.abo7896. 10.1126/science.abo7896 PubMed 35762884
Included in kit:
Commonly requested with:
