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(Plasmid #184463)


Item Catalog # Description Quantity Price (USD)
Plasmid 184463 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Point mutation at base 1963 (A to C), which changes Asn642 to His
  • Entrez Gene
  • Tag / Fusion Protein
    • FLAG Tag (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wild-type mouse STAT5b expression plasmid with an N-terminal Flag tag was obtained from SinoBiological, Wayne PA (cat. # MG51116-NF). A point mutation at base 1963 (A to C), which changes Asn642 to His, was introduced into the STAT5b coding sequence by Genscript (Piscataway, NJ). This mutation confers constitutive activity (CA) to STAT5b (Ono M. et el. Mol Endocrinol. 2007). The mutated plasmid was sent to Penn Vector Core (University of Pennsylvania, Philadelphia, PA), where it was cloned into the Penn Vector Core plasmid pENN.AAV.TBG.PI.eGFP.WPRE.bGH, replacing eGFP by the N-Flag-mSTAT5bCA cDNA. The resultant plasmid, pENN.AAV.TBG.PI.N-FLAG mSTAT5bCA.WPRE.bGH, was sequence verified then packaged.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH was a gift from Rhonda Kineman (Addgene plasmid # 184463 ; ; RRID:Addgene_184463)
  • For your References section:

    Constitutively active STAT5b feminizes mouse liver gene expression. Lau-Corona D, Ma H, Vergato C, Sarmento-Cabral A, Del Rio-Moreno M, Kineman RD, Waxman DJ. Endocrinology. 2022 Apr 9. pii: 6565757. doi: 10.1210/endocr/bqac046. 10.1210/endocr/bqac046 PubMed 35396838