pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH
(Plasmid #184463)

• Purpose: pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.
• Depositing Lab: Rhonda Kineman
• Publication: Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757. doi: 10.1210/endocr/bqac046.

Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7309
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-Flag-mSTAT5bCA
• Alt name: STAT5CA
• Species: M. musculus (mouse)
• Insert Size (bp): 2370
• Mutation: Point mutation at base 1963 (A to C), which changes Asn642 to His
• Entrez Gene: Stat5b
• Tag / Fusion Protein:
  • FLAG Tag (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wild-type mouse STAT5b expression plasmid with an N-terminal Flag tag was obtained from SinoBiological, Wayne PA (cat. # MG51116-NF). A point mutation at base 1963 (A to C), which changes Asn642 to His, was introduced into the STAT5b coding sequence by Genscript (Piscataway, NJ). This mutation confers constitutive activity (CA) to STAT5b (Ono M. et el. Mol Endocrinol. 2007). The mutated plasmid was sent to Penn Vector Core (University of Pennsylvania, Philadelphia, PA), where it was cloned into the Penn Vector Core plasmid pENN.AAV.TBG.PI.eGFP.WPRE.bGH, replacing eGFP by the N-Flag-mSTAT5bCA cDNA. The resultant plasmid, pENN.AAV.TBG.PI.N-FLAG mSTAT5bCA.WPRE.bGH, was sequence verified then packaged.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry