pαH-S-GSAS-OMICRON.BA.1-RBD
(Plasmid #184831)

• Purpose: Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
• Depositing Lab: Priyamvada Acharya
• Publication: Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi: 10.1016/j.celrep.2022.111009. Epub 2022 Jun 8.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4746
• Total vector size (bp): 5498
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S-GSAS-OMICRON.BA.1
• Species: Severe acute respiratory syndrome coronavirus 2
• Insert Size (bp): 816
• Mutation: SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 variant; G339D, S371L, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Tag / Fusion Protein:
  • 6X His tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pCAGGS-5

Resource Information

• Supplemental Documents:
  • RBD OMICRON.BA.1.gb
• A portion of this plasmid was derived from a plasmid made by: F Krammer, F Amanat, S Strohmeier BEI resources (Catalog number NR-52309)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.