pFSW GAP43-jRGECO1a P2A Syp-Halo
(Plasmid
#185565)
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PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFSW
- Backbone size w/o insert (bp) 8400
- Total vector size (bp) 11800
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAP43-jRGECO1a P2A Syp-Halo
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3400
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Tag
/ Fusion Protein
- HaloTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFSW GAP43-jRGECO1a P2A Syp-Halo was a gift from Edwin Chapman (Addgene plasmid # 185565 ; http://n2t.net/addgene:185565 ; RRID:Addgene_185565) -
For your References section:
All-optical monitoring of excitation-secretion coupling demonstrates that SV2A functions downstream of evoked Ca(2+) entry. Bradberry MM, Chapman ER. J Physiol. 2022 Feb;600(3):645-654. doi: 10.1113/JP282601. Epub 2022 Jan 13. 10.1113/JP282601 PubMed 34957569
