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Addgene

pFSW GAP43-jRGECO1a P2A Syp-Halo
(Plasmid #185565)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185565 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFSW
  • Backbone size w/o insert (bp) 8400
  • Total vector size (bp) 11800
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAP43-jRGECO1a P2A Syp-Halo
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3400
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFSW GAP43-jRGECO1a P2A Syp-Halo was a gift from Edwin Chapman (Addgene plasmid # 185565 ; http://n2t.net/addgene:185565 ; RRID:Addgene_185565)
  • For your References section:

    All-optical monitoring of excitation-secretion coupling demonstrates that SV2A functions downstream of evoked Ca(2+) entry. Bradberry MM, Chapman ER. J Physiol. 2022 Feb;600(3):645-654. doi: 10.1113/JP282601. Epub 2022 Jan 13. 10.1113/JP282601 PubMed 34957569