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pCAGGS SARS-CoV-2 Delta Spike
(Plasmid #185593)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 185593 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4717
  • Total vector size (bp) 8529
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Delta Spike
  • Alt name
    SARS-CoV-2 B.1.617.2 Spike
  • Insert Size (bp)
    3810
  • Mutation
    Contains the following mutations: T19R, Δ156-157, R158G, L452R, T478K, D614G, P681R, D950N
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter chicken β-actin promoter, CMV enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CTCTAGAGCCTCTGCTAACCATGTTC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS SARS-CoV-2 Delta Spike was a gift from Marceline Côté (Addgene plasmid # 185593 ; http://n2t.net/addgene:185593 ; RRID:Addgene_185593)
  • For your References section:

    Identification of a SARS-CoV-2 host metalloproteinase-dependent entry pathway differentially used by SARS-CoV-2 and variants of concern Alpha, Delta, and Omicron. Benlarbi M, Laroche GA, Fink C , Fu K , Mulloy RP, Phan A, Ariana A, Stewart CM, Prévost J, Beaudoin-Bussières G , Daniel R, Bo Y, Yockell-Lelièvre J, Stanford WL, Giguère PM, Mubareka S, Finzi A, Dekaban GA, Dikeakos JD, Côté M. bioRxiv 2022.02.19.481107 10.1101/2022.02.19.481107