pMX-53BP1(1-1710) 3LA
(Plasmid
#185698)
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PurposeMammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
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Depositing Lab
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Depositing OrganizationSinai Health System
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 185698-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 185698-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepMX
- Total vector size (bp) 10865
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name53BP1(1-1710) 3LA
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SpeciesH. sapiens (human)
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Insert Size (bp)5130
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MutationL175A, L517A, and L693A
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GenBank IDNM_005657.4 NM_005657.4
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
- Promoter SV40
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Tags
/ Fusion Proteins
- Flag tag (C terminal on insert)
- HA tag (C terminal on insert)
- tetracysteine tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer gcttggatacacgccgc
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note plasmid contains a K1031E mutation in the 53BP1(1-1710) 3LA insert. This mutation does not affect plasmid function.
DNA (Catalog # 185698-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 185698-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX-53BP1(1-1710) 3LA was a gift from Daniel Durocher (Addgene plasmid # 185698 ; http://n2t.net/addgene:185698 ; RRID:Addgene_185698) -
For your References section:
RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1. Setiaputra D, Escribano-Diaz C, Reinert JK, Sadana P, Zong D, Callen E, Sifri C, Seebacher J, Nussenzweig A, Thoma NH, Durocher D. Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24. 10.1016/j.molcel.2022.01.025 PubMed 35216668
