pMX-53BP1(1-1710) 3LA
(Plasmid #185698)

• Purpose: Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
• Depositing Lab: Daniel Durocher
• Publication: Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24.

Backbone

• Vector backbone: pMX
• Total vector size (bp): 10865
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1(1-1710) 3LA
• Species: H. sapiens (human)
• Insert Size (bp): 5130
• Mutation: L175A, L517A, and L693A
• GenBank ID: NM_005657.4 NM_005657.4
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Promoter: SV40
• Tags / Fusion Proteins:
  • Flag tag (C terminal on insert)
  • HA tag (C terminal on insert)
  • tetracysteine tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: gcttggatacacgccgc

Resource Information

• Supplemental Documents:
  • pMX 53BP1(1-1710) 3LA vAddgene.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note plasmid contains a K1031E mutation in the 53BP1(1-1710) 3LA insert. This mutation does not affect plasmid function.