pDONR-53BP1 1-1972 28A
(Plasmid #53447)

• Purpose: Recombinational donor/master vector
• Depositing Lab: Daniel Durocher
• Publication: Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12.

Backbone

• Vector backbone: Gateway pDONR 221
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 4761
• Total vector size (bp): 10677
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1
• Alt name: siRNA resistant and ATM site-deficient construct of 53BP1
• Species: H. sapiens (human)
• Insert Size (bp): 5916
• Mutation: I914T mutation; All 28 SQ/TQ motifs in N-term of 53BP1 have been mutated to Ala; siRNA resistant mutation in 53BP1 is ACGCGCTGACGACCCGCTT
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA