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Integrin Beta1-mPA-GFP
(Plasmid #186302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186302 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 186302-D50 50 μg of DNA in Tris buffer $495
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DNA 186302-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Clontech N1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 7213
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrin Beta 1
  • Alt name
    ITGB1
  • Alt name
    CD29
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2394
  • GenBank ID
    NM_002211.3
  • Entrez Gene
    ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
  • Promoter CMV
  • Tag / Fusion Protein
    • mPA-GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Photoconvertable:
On: Excitation 504 nm, Emission 517 nm
Off to On: 400 nm

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin Beta1-mPA-GFP was a gift from Pakorn Kanchanawong (Addgene plasmid # 186302 ; http://n2t.net/addgene:186302 ; RRID:Addgene_186302)

  • For your References section:

    Ligand functionalization of titanium nanopattern enables the analysis of cell-ligand interactions by super-resolution microscopy. Jain K, Kanchanawong P, Sheetz MP, Zhou X, Cai H, Changede R. Nat Protoc. 2022 Jul 27. pii: 10.1038/s41596-022-00717-3. doi: 10.1038/s41596-022-00717-3. 10.1038/s41596-022-00717-3 PubMed 35896742
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