pQE80_T5_P9-ehaA_LacI
(Plasmid
#186317)
-
PurposePlasmid for inducible E coli expression of the P9 helixCAM.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepQE80
-
Backbone manufacturerQiagen
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEBExpress Iq Competent E coli
-
Growth instructionsGrows in normal cloning strains and conditions. Provided in the NEBExpress Iq strain for ease of expression induction.
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameP9-ehaA
-
SpeciesSynthetic
-
Insert Size (bp)1239
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pQE80_T5_P9-ehaA_LacI was a gift from George Church (Addgene plasmid # 186317 ; http://n2t.net/addgene:186317 ; RRID:Addgene_186317) -
For your References section:
helixCAM: A platform for programmable cellular assembly in bacteria and human cells. Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM. Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. 10.1016/j.cell.2022.08.012 PubMed 36055250
