helixCAM: A platform for programmable cellular assembly in bacteria and human cells.
Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM
Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. PubMed Article
Chao G, Wannier TM, Gutierrez C, Borders NC, Appleton E, Chadha A, Lebar T, Church GM
Cell. 2022 Aug 23. pii: S0092-8674(22)01061-3. doi: 10.1016/j.cell.2022.08.012. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186303 | PB_TRE_Z17-PDGFR_EF1a_mCherry | Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction. |
| 186304 | PB_TRE_Z18-PDGFR_EF1a_eGFP | Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction. |
| 186305 | PB_TRE_P3-PDGFR_EF1a_iRFP670 | Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction. |
| 186306 | PB_TRE_AP4-PDGFR_EF1a_eBFP2 | Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction. |
| 186307 | PB_TRE_P9-PDGFR_EF1a_mPlum | Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction. |
| 186308 | PB_TRE_AP10-PDGFR_EF1a_mOrange | Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction |
| 186309 | PB_TRE_sg30-PDGFR_EF1a_mCherry | Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction |
| 186310 | PB_TRE_sg61-PDGFR_EF1a_eGFP | Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction |
| 186311 | PB_TRE_sg83-PDGFR_EF1a_iRFP670 | Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction |
| 186312 | PB_TRE_sg88-PDGFR_EF1a_eBFP2 | Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction. |
| 186313 | pQE80_T5_Z17-ehaA_LacI | Plasmid for inducible E coli expression of the Z17 helixCAM. |
| 186314 | pQE80_T5_Z18-ehaA_LacI | Plasmid for inducible E coli expression of the Z18 helixCAM. |
| 186315 | pQE80_T5_P3-ehaA_LacI | Plasmid for inducible E coli expression of the P3 helixCAM. |
| 186316 | pQE80_T5_AP4-ehaA_LacI | Plasmid for inducible E coli expression of the AP4 helixCAM. |
| 186317 | pQE80_T5_P9-ehaA_LacI | Plasmid for inducible E coli expression of the P9 helixCAM. |
| 186318 | pQE80_T5_AP10-ehaA_LacI | Plasmid for inducible E coli expression of the AP10 helixCAM. |