pQCXIP EGFP-ER [TM(SAC1)]
(Plasmid #186475)

• Purpose: Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells
• Depositing Lab: Catherine Tomasetto
• Publication: Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-12. doi: 10.1242/jcs.139295. Epub 2013 Oct 8.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6991
• Total vector size (bp): 7957
• Modifications to backbone: Replacement of the puromycin resistance gene by a blasticidine resistance gene
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAC1
• Species: H. sapiens (human)
• Insert Size (bp): 204
• Mutation: C-terminal transmembrane region of the protein: 521-587
• GenBank ID: XM_011533500.3
• Entrez Gene: SACM1L (a.k.a. SAC1)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Tamas Balla

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Visualization and manipulation of plasma membrane-endoplasmic reticulum contact sites indicates the presence of additional molecular components within the STIM1-Orai1 Complex. Péter Várnai, Balázs Tóth, Dániel J Tóth, László Hunyady, Tamas Balla
J Biol Chem. 2007 Oct 5;282(40):29678-90. PMID: 17684017 DOI: 10.1074/jbc.M704339200