MARCKS(1-40)-paGFP
(Plasmid
#18695)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMARCKS(1-40)
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SpeciesH. sapiens (human)
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Insert Size (bp)120
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MutationA206K mutation in paGFP
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GenBank IDDQ341274
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Entrez GeneMARCKS (a.k.a. 80K-L, MACS, PKCSL, PRKCSL)
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Tag
/ Fusion Protein
- photoactivatable GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJ. Lippincott-Schwartz (paGFP)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MARCKS(1-40)-paGFP was a gift from Karel Svoboda (Addgene plasmid # 18695 ; http://n2t.net/addgene:18695 ; RRID:Addgene_18695)