MARCKS(1-40)-paGFP
(Plasmid #18695)

Depositing Lab

Karel Svoboda
Publication

Spatial spread of Ras activity (unpublished) ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	18695	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pRK5
(Search Vector Database)
Backbone size w/o insert (bp) 4700
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
MARCKS(1-40)
Species
H. sapiens (human)
Insert Size (bp)
120
Mutation
A206K mutation in paGFP
GenBank ID
DQ341274
Entrez Gene
MARCKS (a.k.a. 80K-L, MACS, PKCSL, PRKCSL)
Tag / Fusion Protein
- photoactivatable GFP (C terminal on insert)

Cloning Information

Cloning method Restriction Enzyme
5′ sequencing primer CMV Forward

(Common Sequencing Primers)

Resource Information

A portion of this plasmid was derived from a plasmid made by
J. Lippincott-Schwartz (paGFP)

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

MARCKS(1-40)-paGFP was a gift from Karel Svoboda (Addgene plasmid # 18695 ; http://n2t.net/addgene:18695 ; RRID:Addgene_18695)

Map uploaded by the depositor.

MARCKS(1-40)-paGFP (Plasmid #18695)