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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD/Myc-HisC
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4093
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepiggyBac transposase
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Alt nameT.ni piggyBac transposase ORF
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SpeciesTrichoplusia ni
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Insert Size (bp)1782
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MutationN/A
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GenBank IDDQ340395
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Tags
/ Fusion Proteins
- Myc (C terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer gattagcggatcctacctg
- 3′ sequencing primer gacggcgctattcagatc (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDavid A. O'Brochta, Ph.D Professor Center for Biosystems Research University of Maryland Biotechnology Institute Room 5141 Plant Sciences Building College Park, MD 20742 Phone: 301-405-7680 Fax: 301-314-9075 Email :[email protected]
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p-transposase was a gift from Nancy Craig (Addgene plasmid # 18699) -
For your References section:
piggyBac can bypass DNA synthesis during cut and paste transposition. Mitra R, Fain-Thornton J, Craig NL. EMBO J. 2008 Apr 9. 27(7):1097-109. 10.1038/emboj.2008.41 PubMed 18354502