Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

p-transposase
(Plasmid #18699)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18699 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD/Myc-HisC
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4093
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    piggyBac transposase
  • Alt name
    T.ni piggyBac transposase ORF
  • Species
    Trichoplusia ni
  • Insert Size (bp)
    1782
  • Mutation
    N/A
  • GenBank ID
    DQ340395
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer gattagcggatcctacctg
  • 3′ sequencing primer gacggcgctattcagatc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David A. O'Brochta, Ph.D Professor Center for Biosystems Research University of Maryland Biotechnology Institute Room 5141 Plant Sciences Building College Park, MD 20742 Phone: 301-405-7680 Fax: 301-314-9075 Email :[email protected]

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-transposase was a gift from Nancy Craig (Addgene plasmid # 18699)
  • For your References section:

    piggyBac can bypass DNA synthesis during cut and paste transposition. Mitra R, Fain-Thornton J, Craig NL. EMBO J. 2008 Apr 9. 27(7):1097-109. 10.1038/emboj.2008.41 PubMed 18354502