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pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFP
(Plasmid #187071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187071 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDNA5/FR/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B_dHaloTag7
  • Alt name
    H2B_dHaloTag7
  • Alt name
    mEGFP
  • Species
    H. sapiens (human)
  • Mutation
    dHaloTag7 = HaloTag7-D106A

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFP was a gift from Kai Johnsson (Addgene plasmid # 187071 ; http://n2t.net/addgene:187071 ; RRID:Addgene_187071)

  • For your References section:

    Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy. Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, D'Este E, Heilemann M, Hiblot J, Johnsson K. J Am Chem Soc. 2023 Feb 8;145(5):3075-3083. doi: 10.1021/jacs.2c11969. Epub 2023 Jan 30. 10.1021/jacs.2c11969 PubMed 36716211
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